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Single Nucleotide Variation Detection on 3D DNA Microarray by Ligation of Two-Terminal-Modified Universal Probes

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Exploiting the advantages of three-dimensional (3D) DNA microarray, we put forward a novel strategy termed "ligation of two-terminal-modified universal probes" for single nucleotide variation (SNV) detection on 3D microarray. By performing specific ligation reaction between the unmodified hybridization primer with 3' hydroxyl terminus and the universal probe with phosphorylated 5' terminus and fluorescently labeled 3' terminus, two point mutations (C3206T and A5301G) in the PCR products immobilized on the 3D polyacrylamide gel DNA microarray were accurately discriminated. This method can not only maintain the predominance of 3D DNA microarray as a platform with high through-put, but also can exert this predominance to the cases of detecting a small quantity of samples and multiple SNVs since four universal probes can be employed to detect all SNVs, therefore, it is more feasible for laboratory research and provide an effective tool for clinical diagnosis.
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Document Type: Research Article

Publication date: 2011-09-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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