@article {Sharma:2011:1533-4880:7549,
title = "Superior Neuroprotective Effects of Cerebrolysin in Heat Stroke Following Chronic Intoxication of Cu or Ag Engineered Nanoparticles. A Comparative Study with Other Neuroprotective Agents Using Biochemical and Morphological Approaches in the Rat",
journal = "Journal of Nanoscience and Nanotechnology",
parent_itemid = "infobike://asp/jnn",
publishercode ="asp",
year = "2011",
volume = "11",
number = "9",
publication date ="2011-09-01T00:00:00",
pages = "7549-7569",
itemtype = "ARTICLE",
issn = "1533-4880",
eissn = "1533-4899",
url = "https://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000009/art00003",
doi = "doi:10.1166/jnn.2011.5114",
keyword = "BLOOD-BRAIN BARRIER, PREGABALIN, HEAT STRESS, CEREBROLYSIN, VALPROATE, HEAT STROKE, BRAIN EDEMA, NANOPARTICLES, HYPERTHERMIA, NEUROPROTECTION, TOPIRAMATE, NEURONAL INJURIES, LEVETIRACETAM",
author = "Sharma, Hari Shanker and Muresanu, Dafin Fior and Patnaik, Ranjana and Stan, Adina Dora and Vacaras, Vitalie and Perju-Dumbrava, Laura and Alexandru, Badisor and Buzoianu, Anca and Opincariu, Iulian and Menon, Preeti Kumaran and Sharma, Aruna",
abstract = "The possibility that cerebrolysin, a mixture of several active fragments of neurotrophic factors and peptides induces neuroprotection following nanoparticles induced exacerbation of brain damage in heat stroke was examined in a rat model. For this purpose, the therapeutic efficacy of
Cerebrolysin (2.5 or 5 ml/kg) recommended for stroke treatment was used in comparison with other drugs in standard doses recommended for such therapy in clinical situations e.g., levetiracetam (44 mg/kg), pregabalin (200 mg/kg), topiramate (40 mg/kg, i.p.) and valproate (400 mg/kg). Rats subjected
to 4 h heat stress in a biological oxygen demand (BOD) incubator at 38 \textdegreeC (Rel Humid 4547%; Wind vel 22.4 to 25.6 cm/sec) developed profound behavioral symptoms of heat stroke e.g., hyperthermia, profuse salivation, prostration and gastric ulcerations in the stomach. These rats
also exhibited marked brain pathology at this time. Thus, breakdown of the blood-brain barrier (BBB) to proteins associated with brain edema formation could be seen in these heat stressed rats as compared to control groups. The edematous brain areas showed profound neuronal damage and/or distortion
in large areas of the neuropil. These pathological symptoms were further exacerbated in Cu or Ag nanoparticles treated group (5060 nm particle size, 50 mg/kg, i.p./day for 7 days) after identical heat stress on the 8th day. Pretreatment with cerebrolysin (2.5 ml/kg, i.v.) daily for
3 days in normal rats before heat stress significantly reduced the behavioral stress symptoms and the breakdown of the BBB function, edema formation and neuronal injuries. However, the magnitude and intensity of these neuroprotective effects were much less intense in all other drug treated
rats after similar heat stress. On the other hand, almost double dose of cerebrolysin (5 ml/kg) was needed to achieve comparable neuroprotection in nanoparticles treated animals after heat stress. Whereas, double dose of all other compounds was much less effective in inducing neuroprotection
in nanoparticles treated heat-exposed animals. These observations are the first to show that cerebrolysin exerts the most superior neuroprotective effects in heat stress as compared to other neuroprotective agents on brain pathology in normal and in nanoparticles treated group. Furthermore,
cerebrolysin in double dose was the most effective in inducing neuroprotection in nanoparticles treated heat exposed rats on brain pathology as compared to double doses of other drugs. Taken together, our results show that cerebrolysin has the most superior neuroprotective effects on brain
pathology in heat stroke in both normal and nanoparticles treated rats as compared to other contemporary neuroprotective agents, not reported earlier.",
}