Phenanthroline-Based Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

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Abstract:

We herein report the preparation of PSMN (phenanthroline-based silica coated magnetic nanoparticles), and their applications for protein purification as selective magnetic probes of histidine-tagged proteins in cell lysates. This simple system serves as a useful alternative to existing protocols for his-tagged protein separation and as a versatile agent for transporting and anchoring proteins.

Keywords: HISTIDINE-TAGGED PROTEINS; MAGNETIC NANOPARTICLE; PHENANTHROLINE

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2011.4869

Publication date: August 1, 2011

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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