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Magnetic/Polymer/Nanogold Complex Using as a Novel Enzyme Support

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A novel enzyme support based on magnetic/polymer/nanogold complex was prepared in this study. At first, carboxymethyl chitosan (CMC) were connected onto the surface of Fe3O4 nanoparticles, and then gold nanoparticles were in situ synthesized and bound to CMC chains, resulting in Fe3O4/CMC/nanogold complex. The complex was determined by UV-vis spectroscopy, transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Horseradish peroxidase (HRP) was immobilized on the Fe3O4/CMC/nanogold complex. Compared with free HRP, the immobilized HRP exhibits higher catalytic activity and better storage stability. This Fe3O4/CMC/nanogold complex can serve as an excellent support for enzymes.
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Keywords: CARBOXYMETHYL CHITOSAN; GOLD NANOPARTICLES; HRP; MAGNETIC NANOPARTICLES

Document Type: Research Article

Publication date: 01 June 2011

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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