Skip to main content

Detection of the Effect of Environmental Toxicants on the Synthetic Peptide Modified Neural Cell Chip

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

A cell based chip was designed to differentiate and to detect the effects of environmental chemicals on the neurite outgrowth in PC12 cell. To fabricate platform of cell chip, gold surfaces were modified by RGD based synthetic oligopeptide. Nanoscale controlled self-assembled peptide layer was investigated by Atomic Force Microscopy (AFM). On the fabricated cell chip, PC12 cell was immobilized and the differentiation of neurite outgrowth in PC12 cells was done by neurite growth factor (NGF). Differentiation of PC12 cell was confirmed by immunofluorescence study. Further the differentiation and the length of neurite was confirmed by confocal microscopy study. Voltammetry behavior of the neurite induced PC12 and the electrochemical behavior of the environmental toxicants effect on the neurite outgrowth was measured by cyclic voltammetry (CV). Self-assembled layer mediated cell immobilization technique and voltammetric signal analysis system can be applied to construct the neural cell chip for the detection of large number of environmental toxins and various neurotoxicants.

Keywords: ATOMIC FORCE MICROSCOPY; CYCLIC VOLTAMMETRY; ENVIRONMENTAL TOXIN; NANOBIOCHIP; NEURAL CELL CHIP

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2011.3672

Publication date: May 1, 2011

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
asp/jnn/2011/00000011/00000005/art00145
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more