Influence of Diquat on Growth and Death of HepG2 Cells Using Quartz Crystal and Micro CCD Camera
Abstract:Diquat is widely used agent which produces toxicity in human and implicated as an environmental toxicity. HepG2 cell was cultured onto an indium tin oxide (ITO) surface of quartz crystal modified a collagen film. In this paper, we investigated the physical properties and the morphological change of the HepG2 cells cultured onto the ITO electrode of the quartz crystal sensor with micro CCD camera. The resonance responses of the quartz crystal and the morphological change were directly monitored. After seeding the cells and diquat injection into the chamber, the resonance frequency and the resonance resistance were obtained with real time morphologies. From the resonance characteristics and the series of morphologies, we could know the diquat to be death and weakening of the cells.
Document Type: Research Article
Publication date: 2011-05-01
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