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Nanoscale Fabrication of Myoglobin Monolayer on Self-Assembled DTSSP for Bioelectronic Device

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Abstract:

The fabrication method of nanoscale myoglobin monolayer using chemical linker is introduced in this study because control of amount and orientation of protein immobilized on electronic device is one of main issues to be solved for the realization of biomolecular electronic device. Myoglobin, metalloprotein, is selected as active material due to its electrochemical property. To immobilize myoglobin on Au surface, 3,3-dithiobis (sulphosuccinimidyl propionate) (DTSSP) is utilized as a chemical linker. The optimum amount of protein is investigated by surface plasmon resonance (SPR). SPR and scanning tunneling microscope (STM) results confirm the nano scale protein layer formed on DTSSP self assembled monolayer (SAM) on Au surface. Protein layer on Au surface using DTSSP as chemical linker was more stable than random adsorption without linker as aspect of redox character due to the fact that myoglobin immobilized with chemical linker did not lose its redox property after long usages.

Keywords: CYCLIC VOLTAMMETRY; MYOGLOBIN; NANOBIOCHIP; SCANNING TUNNELING MICROSCOPY; SURFACE PLASMON RESONANCE

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2011.3665

Publication date: 2011-05-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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