Authors: Choi, Jeong-Woo; Islam, A. T. M. Kamrul; Lee, Jin-Ho; Song, Joon Myong; Oh, Byung-Keun

Source: Journal of Nanoscience and Nanotechnology, Volume 11, Number 5, May 2011 , pp. 4200-4204(5)

Publisher: American Scientific Publishers

Abstract:

In this study an immuno-array for A 42 based on scanning tunneling microscopy (STM) was developed using conjugated gold nanoparticle (Au-NP) and antibody (Ab) complex. Fragmented monoclonal Ab against Aβ42 was allowed to immobilize on the Au-dot arrays followed by its target protein Aβ42 and Au NP and Ab complex. The surface structure of Au-NP and Ab complex on Au-dots was investigated with Atomic Force Microscopy and the current profile of fabricated immunosensing element was investigated with STM. The power spectrum derived from the current profile was found to be increasing with higher concentrations of Aβ42 having a detection limit of 100 fg/ml. The proposed technique can be a promising method to construct the highly sensitive and efficient protein chip of immunosensors arrays.

Keywords: ALZHEIMER'S DISEASE; BETA-AMYLOID (1-42); SCANNING TUNNELING MICROSCOPY; AU NANO PARTICLE; NANOBIOCHIP

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2011.3673

Publication date: 2011-05-01

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