Authors: Yi, Xianghua; Ding, Yongmei; Zeng, Yu; Zhou, Caicun; Luo, Benfang; Meng, Shuyan; Rui, Weiwei; Zhao, Yinmin; Li, Wei

Source: Journal of Nanoscience and Nanotechnology, Volume 11, Number 5, May 2011 , pp. 3800-3807(8)

Publisher: American Scientific Publishers

Abstract:

To construct tumor-targeted nanometer particles as a negative magnetic resonance imaging (MRI) contrast agent. Ultra-small superparamagnetic iron oxide (USPIO) nanometer particles were prepared by one-step chemical precipitation. The covalent bond between cyclic RGD (cRGD) containing an Arg-Gly-Asp sequence targeting integrin-αvβ3, and USPIO was conducted by chemical crosslinking. The physico-chemical property of cRGD-USPIO was detected. Prussian blue staining was applied to detect the specific binding capacity of cRGD-USPIO and USPIO to human pulmonary adenocarcinoma A549 cells and human umbilical vein endothelial cells. Subsequently, A549 xenografts in nude mice were established, and intravenous injections of USPIO and cRGD-USPIO into the vena caudalis were performed. The enhancement of cRGD-USPIO against tumor MRI signal was evaluated. The mean hydrodynamic diameter of cRGD-USPIO was 43.97 ± 10.10 nm and the size of the ferric oxide core was 5∼10 nm. The specific saturation magnetization was 59.94 A · m² · Kg⁻¹. The cell conjugation assay results indicated that the positive staining of the cRGD-USPIO group was significantly enhanced. The in vivo MRI diagnosis indicated that the cRGD-USPIO tumor signal was significantly reduced compared to that of the USPIO group (P < 0.01). The targeted superparamagnetic iron oxide nanometer particle can be a novel MRI negative contrast agent for more specific tumor early diagnosis.

Keywords: MAGNETIC RESONANCE IMAGING; NANOMETER TECHNOLOGY; ULTRASMALL SUPERPARAMAGNETIC IRON OXIDE; CARCINOMA; EARLY DIAGNOSIS

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2011.3861

Publication date: 2011-05-01

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