Provider: Ingenta Connect
Database: Ingenta Connect
Content: application/x-research-info-systems
TY - ABST
AU - Wang, Yajie
AU - Wang, Yanming
AU - Wang, Ling
AU - Che, Yongzhe
AU - Li, Zongjin
AU - Kong, Deling
TI - Preparation and Evaluation of Magnetic Nanoparticles for Cell Labeling
JO - Journal of Nanoscience and Nanotechnology
PY - 2011-05-01T00:00:00///
VL - 11
IS - 5
SP - 3749
EP - 3756
KW - CELL LABELING
KW - MAGNETIC IRON OXIDE NANOPARTICLES
KW - MESENCHYMAL STEM CELLS
KW - CELL TRACKING
N2 - Cell labeling and tracking are becoming increasingly important in the fields of stem cell transplantation. To track the migration and distribution of the implanted cells is critical for understanding the beneficial effects of stem cell therapy. The aim of this study is to synthesize
new superparamagnetic nanoparticles and investigate the feasibility of magnetic labeling of bone marrow mesenchymal stem cells (MSCs). Monodisperse hydrophobic magnetite (Fe3O4) nanoparticles were prepared through high temperature decomposition of Fe(acac)3
and a long-chain alcohol. The nanoparticles were further modified with a bipolar surfactant, 2,3-dimercaptosuccinic acid (DMSA) and then transformed into water-soluble iron oxide nanoparticles (WION). The magnetic particles showed uniform size (10 nm), high efficiency and stability in MSCs
labeling. The labeled cells were cultured until passage 8, there is no reduction in magnetic tropism and the percentage of labeled cells. The results of MTT, proliferation assay and flow cytometry analysis show that the WION are biocompatible. The labeling process does not cause cell death
and apoptosis, and has no side effect on growth capacity of the cells. In conclusion, the successful and stable labeling of MSCs and the efficient magnetic tropism indicate that this WION can be used for tracking of MSCs in future MSCs-based stem cell therapy.
UR - https://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000005/art00003
M3 - doi:10.1166/jnn.2011.3822
UR - https://doi.org/10.1166/jnn.2011.3822
ER -