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Metabonomic Studies of Biochemical Changes in the Serum of Rats by Intratracheally Instilled TiO2 Nanoparticles

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In vivo behavior of nanoparticles and interactions between nanoparticles and organisms are key components of nanotoxicology. In this study, the biochemical compositions of rat serum exposed to TiO2 nanoparticles (nano-TiO2) by intratracheal instillation at low (0.8 mg/kg), medium (4 mg/kg) and high doses (20 mg/kg) were investigated using 1H-NMR techniques with pattern recognition methods. Serum biochemical assays, as well as histopathological and transmission electron microscopy (TEM) examinations of tissues were performed. 1H-NMR spectra and pattern recognition analyses of nano-TiO2 treated animals showed increased serum levels of ketone bodies, choline, low density lipoprotein (LDL), alanine and GLU, and decreased levels of lactate, creatine and pyruvate. Clinical chemistry analyses of serum showed increased levels of lactate dehydrogenase, aspartate aminotransferase, and alkaline phosphatase, as well as increased levels of blood urea nitrogen and creatinine, indicating slight liver and kidney injury. Furthermore, TEM provided an analysis of particle-related structural changes of the lungs, liver and kidneys, and revealed that the localization of nanoparticles within cells induced apoptosis. The damage to the target organs was associated with the perturbation of energy metabolism. Serum 1H-NMR spectra, in combination with pattern recognition techniques, proved to be an efficient and simple metabonomic method to depict the metabolic changes produced by intratracheally-instilled nano-TiO2.
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Keywords: METABONOMICS; NANOPARTICLE; NANOTOXICITY; SERUM; TIO2

Document Type: Research Article

Publication date: 2011-04-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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