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2-Color Encoding Solid Phase Minisequencing

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Solid phase minisequencing is an effective single nucleotide determination technique. However, two main methods currently used are both time and money consuming. Here, we introduced a more economical and time efficient approach, called 2-color encoding minisequencing, in which four varieties of nucleotides were labeled by the combination of two fluorescence dyes. Such code was sensitive for different nucleotides, and the sequencing results were highly uniform and repeatable.
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Keywords: 2-COLOR ENCODING; DNA MICROARRAY; MINISEQUENCING; SINGLE NUCLEOTIDE POLYMORPHISMS; SNPS

Document Type: Research Article

Publication date: 2011-03-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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