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Carboxyl-Functionalized Superparamagnetic Fe3O4/Poly(St-co-MPS)/SiO2 Composite Particles for Rapid and Sensitive Immunoassay

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Superparamagnetic Fe3O4/poly(St-co-MPS)/SiO2 composite particles with the average size of 140 nm were functionalized with carboxyl group by emulsion polymerization. Functionalized particles with carboxyl contents of 13.6 and 136 μmol/mL were prepared by changing the amount of acrylic acid monomer used in the polymerization. After conjugation with human chorionic gonadotrophin (hCG) antibody, the particles were used to construct lateral flow immunoassays (LFIA) for the detection of hCG in solution. The quantitative analysis could be finished in 20 min by using the magnetic particles as labels. The detection limit of LFIA was determined to be 1 and 5 IU/L respectively for the magnetic particles with carboxyl contents of 13.6 and 136 μmol/mL.
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Document Type: Research Article

Publication date: 01 March 2011

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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