Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro
Authors: Kim, Tae-Hun; Yu, Gwang Sig; Choi, Hye; Shim, Young Jung; Lee, Minhyung; Choi, Joon Sig
Source: Journal of Nanoscience and Nanotechnology, Volume 11, Number 2, February 2011 , pp. 1799-1802(4)
Publisher: American Scientific Publishers
Abstract:In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. To make a liposomal solution for gene delivery, DOPE was used as a fusogenic helper lipid. In gel retardation assay, PAMAM-dexamethasone conjugate (PAM-Dex)/DOPE liposome/DNA complex was completely retarded at 8:1 N/P (nitrogen/phosphate) ratio. The physicochemical characteristics are studied by measuring the average size distribution and -potential values of the complexes. In vitro transfection assay showed that the PAM-Dex/DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/DOPE liposome showed the lowest toxicity compared to PAMAM, PEI 25 kD and Lipofectamine. These results indicate that PAM-Dex/DOPE liposome has a potential to be used as an efficient gene carrier for gene therapy.
Document Type: Research Article
Publication date: February 1, 2011
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