Synthesis of Silica-Coated AgI Nanoparticles and Immobilization of Proteins on Them
Abstract:This paper describes a method for preparing silica-coated silver iodide (AgI/SiO2) particles and immobilizing proteins on the AgI/SiO2 particles. Colloid solution of AgI particles was prepared by mixing AgClO4 aqueous solution and KI aqueous solution. Silica-coating of the AgI particles was performed by adding 3-mercaptopropyltrimethoxysilane, tetraethylorthosilicate/ethanol solution and NaOH aqueous solution successively to the AgI colloid solution. TEM observation revealed that the AgI nanoparticles were coated with uniform silica shell. The AgI/SiO2 particles were surface-modified with 3-aminopropyltrimethoxysilane and succinic anhydride. It was confirmed by XPS measurement that amino group or carboxyl group was introduced onto the AgI/SiO2 particles. Protein-immobilization was performed in aqueous solution with bovine serum albumin (BSA) in the presence of the surface-modified AgI/SiO2 particles. UV-VIS absorption spectroscopy revealed that the BSA was adsorbed on the surface-modified particles.
Document Type: Research Article
Publication date: November 1, 2010
More about this publication?
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- ingentaconnect is not responsible for the content or availability of external websites