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Math1 Gene Transfer Based on the Delivery System of Quaternized Chitosan/Na-Carboxymethyl--Cyclodextrin Nanoparticles

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Abstract:

Mammalian cochlear hair cells don't regenerate naturally after injury, which usually leave permanent hearing loss. Math1 gene is a positive regulator of hair cell differentiation during cochlear development and was proved to be very critical in hair cell regeneration in deaf animals. Generating new cochlear hair cells by forced Math1 expression may be a cure for hearing loss. However, satisfying gene delivering vectors in gene therapy are not available. We combined quaternized chitosan (QCS) with Na-carboxymethyl--cyclodextrin (CM--CD) as novel non-viral vector, which adsorbs pRK5-Math1-EGFP perfectly at the mass ratio of 4:1. In vitro cell transfection can reach a 40% transfect efficiency and relatively low cytotoxity than liposomes. These results suggest that QCS/CM--CD nanoparticle complexes could be a novel non-viral gene carrier in further clinical application.

Keywords: CELL TRANSFECT; MATH1 GENE; NANOPARTICLES; QUATERNIZED CHITOSAN/NA-CARBOXYMETHYL-BETA-CYCLODEXTRIN

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2822

Publication date: November 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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