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Aldehyde-Functionalized, Water-Soluble Poly(para-phenylene): Synthesis and Streptavidin Assay Using FRET

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Abstract:

We have attempted to synthesize water-soluble poly(para-phenylene) derivative, poly{[2,5-bis(3-sulfonatobutoxy)-1,4-phenylene sodium salt]-alt-(1,4-phenylene)} (PPP-SO3). Aldehyde groups, versatile functional intermediate groups for immobilization of biomolecules, were introduced at both ends of PPP-SO3 chain to produce PPP-SO3-CHO. PPP-SO3-CHO showed good solubility in aqueous solution and blue emission color, which was expected as an energy donor in FRET mechanism. Biotin was attached to the polymer end groups via imine linkage to use as a ligand for streptavidin immobilization. The biotin coupled with polymer chain enables the polymer to bind with FITC-streptavidin leading to energy transfer from the blue-emitting polymer to green-emitting FITC via FRET.

Keywords: FLUORESCENCE RESONANCE ENERGY TRANSFER; PROTEIN-LIGAND INTERACTION; STREPTAVIDIN ASSAY; WATER-SOLUBLE CONJUGATED POLYMER

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2967

Publication date: October 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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