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Submicron-Patterned Fibronectin Controls the Biological Behavior of Human Dermal Fibroblasts

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Abstract:

Cell adhesion is an important step in cell survival, and in the proliferation of anchorage-dependent cells, whose dimensions can be controlled by micro-patterning of the cell-adhesive extracellular matrix. To fabricate a micro-patterned fibronectin substrate with spacings ranging from 0.9 m to 20 m, we made a replica mold using e-beam lithography. The physiological behavior of human dermal fibroblasts (HDFs) on a substrate with a gradient of pattern spacings from 0.9 m to 20 m was evaluated after 4.5 hours and 2 days of culture. The number of proliferating cells on the fibronectin-patterned surface increased as the spacing between strip lines increased to 11 m. However, the number of cells gradually decreased when the pattern spacing exceeded 11 m. These findings demonstrate that the submicron-patterned topography of a substrate plays important roles in HDF survival and proliferation.

Keywords: FIBRONECTIN; HUMAN DERMAL FIBROBLAST; SELF-ASSEMBLED MONOLAYER; SOFT LITHOGRAPHY; SUBMICRON-PATTERN

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2989

Publication date: October 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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