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Polyaniline–Carboxymethyl Cellulose Nanocomposite for Cholesterol Detection

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Abstract:

Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline–carboxymethyl cellulose (PANI–CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electrochemical studies have been used to characterize the PANI–CMC/ITO nanocomposite electrode and ChOx/PANI–CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI–CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI–CMC/ITO bioelectrode exhibits linearity as 0.5–22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (Km) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI–CMC/ITO nanocomposite electrode.

Keywords: BIOSENSOR; CARBOXYMETHYL CELLULOSE; CHOLESTEROL; CHOLESTEROL OXIDASE; ELECTROCHEMICAL POLYMERIZATION; POLYANILINE

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2511

Publication date: October 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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