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Picomolar Detection Limit on a Magnetoresistive Biochip After Optimization of a Thiol-Gold Based Surface Chemistry

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Abstract:

The surface biochemistry plays a crucial role in the development of stable and reproducible bioanalytical devices. Very often, it represents the bottleneck of a successful integration of magneto-electronic transducers with the biological receptors on its interface. Here is discussed how a thiol-gold surface chemistry can be tailored and optimized in order to allow the biofunctionalization of a magnetoresistive biochip, preventing loss of viability by corrosion while improving its sensitivity. Two important parameters, type of buffer solution and salt concentration (globally ionic strength), were evaluated in the effectiveness of the sulfur-gold linkage and further influence on the biomolecular recognition between single stranded DNA molecules. A third, not less important variable under investigation was the blocking solution. Non-specific adsorption of magnetic labels to the sensing surface still is a major problem to be addressed. The effect of two well known blocking molecules (bovine serum albumin (BSA)) and thiolated polyethylene-glycol (SH-PEG)) on the prevention of non-specific adsorption of targets and labels are compared. The best conditions were selected using an optical microscopic characterization method. Optical images were analyzed for magnetic particles quantification and results presented as a percentage of surface coverage. The optimized protocol was further implemented on real magnetoresistive devices to assess its electric compatibility and bioassay performance. A good reproducibility (about 9% error) among different devices measuring the same target concentration was achieved. Also a reduced non-specific binding signal of 43 V for non-complementary targets (30% complementarity) compares with a 500 V for fully complementarity. A linear range on the biological detection of magnetically labeled target ssDNA oligonucleotides is demonstrated. Consequently, the limit of detection at the standard operational conditions of the device is situated at the picomolar range.

Keywords: MAGNETORESISTIVE BIOCHIP; SURFACE CHEMISTRY

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2010.2595

Publication date: 2010-09-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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