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Production of Poly--Glutamic Acid by Bacillus subtilis and Bacillus licheniformis with Different Growth Media

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Abstract:

Poly -glutamic acid is a naturally occurring homo-polyamide that is made of D- and L-glutamic acid units connected by amide linkages between α-amino and -carboxylic groups. Many strains have been studied for the production of poly -glutamic acid, B. licheniformis 9945a and B. subtilis natto are the two most widely studied strains due to their higher yield. Both of these strains require L-glutamic acid for poly -glutamic acid production. This paper describes investigations on the biosynthesis of poly -glutamic acid by B. licheniformis 9945a and B. subtilis natto in an automated pH-controlled fermenter. The effect of the growth media such as medium E, F, C and GS was investigated. Medium E produced poly -glutamic acid both with and without MnSO4 in the medium when fermented by B. licheniformis. Presence of MnSO4 in the medium affected both the yield and biomass production. The yield was 12 g/l for the medium containing 2.46 mM MnSO4 whereas it was only 4–5 g/l for the medium without MnSO4 with molecular weight (Mw) of 321,000. Different media such as medium C, F and GS were used for production of poly -glutamic acid by B. subtilis natto. The yield from GS medium was highest and it produced about 26–28 g/l with molecular weight (Mw) of 627,000.

Keywords: BACILLUS; BIODEGRADABLE; BIOPOLYMER; FERMENTATION; GLUTAMIC ACID; POLY-GAMMA-GLUTAMIC ACID

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2614

Publication date: September 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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