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Selective Protein Adsorption on ZnO Thin Films for Biofunctional Nano-Platforms

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The immobilization of albumin and lysozyme by spontaneous adsorption on ZnO films, deposited by metal-organic chemical vapour deposition (MOCVD), has been investigated. Quartz crystal microbalance with dissipation monitoring and X-ray photoelectron spectroscopy analyses show that, at physiological pH, the two proteins exhibit different adsorption behaviours, namely albumin irreversibly adsorbs up to coverage of a half of monolayer, while lysozyme does not. Indeed, the high isoelectric point (IEP) of ZnO favors immobilization of biomolecules with lower IEP, assisted by electrostatic attraction in the proper pH range. This selective protein adsorption behaviour results very promising for ZnO nanoplatforms, consisting of hexagonally patterned ZnO nanoring arrays and SiO2 areas, obtained by colloidal template-catalyst assisted MOCVD.


Document Type: Short Communication


Publication date: 2010-09-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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