Nanoemulsion-Eicosapentaenoic Acid Enhanced Alkaline Phosphatase, Calcium Contents, and Surface Molecules Expression During Osteogenesis Using Mouse Multipotent Bone Marrow Stromal Cells

Authors: Kim, Hyung Keun; Park, Sang Jin; Kim, Ji Hyun; Kim, Jin; Lee, Ki-Young; Yoon, Taek Rim

Source: Journal of Nanoscience and Nanotechnology, Volume 10, Number 5, May 2010 , pp. 3284-3288(5)

Publisher: American Scientific Publishers

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Abstract:

Long-chain eicosapentaenoic acid (EPA) is found mainly in marine oils, and has wide ranging biological effects. In this study, we evaluated the effect of nanoemulsion-EPA on the osteogenic differentiation of mouse mesenchymal stem cells (D1 cells). D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days and treated with nanoemulsion-EPA for 2 days and then subjected to various tests; MTT assays, alizarin red S staining, alkaline phosphatase (ALP) activity determinations, calcium contents analysis, reverse transcriptase-polymerase chain reaction (RT-PCR), confocal microscopy, and flow cytometric analysis. D1 cells were found to differentiate into osteoblasts in the presence of osteogenic differentiation medium, as determined by positive alizarin red S staining, increased alkaline phosphatase activity and calcium contents, osteocalcin, osteopontin and Runx2 mRNA expressions, and by positive immunofluorescence staining against CD44 or CD51. Furthermore, osteogenic differentiation was enhanced when cells were treated with nanoemulsion-EPA, as determined by alizarin red S staining, elevated ALP activity, calcium contents and osteocalcin and Runx2 mRNA expression, and immunofluorescence staining and flow cytometric analysis for CD44. Furthermore, pretreatment with p38 inhibitor prior to nanoemulsion-EPA decreased alizarin red S staining and ALP activity. These findings suggest that nanoemulsion-EPA enhances osteogenic differentiation via the p38 pathway when administered to mouse mesenchymal stem cells in osteogenic differentiation medium.

Keywords: ALKALINE PHOSPHATASE; CALCIUM; CD44; CD51; NANOEMULSION-EICOSAPENTAENOIC ACID

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2010.2234

Publication date: May 1, 2010

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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