Authors: Si, Hua-Yan; Li, Dong-Peng; Wang, Tian-Ming; Zhang, Hao-Li; Ren, Fang-Yuan; Xu, Zhu-Guo; Zhao, Yu-Yuan

Source: Journal of Nanoscience and Nanotechnology, Volume 10, Number 4, April 2010 , pp. 2325-2331(7)

Publisher: American Scientific Publishers

Abstract:

The practical application of genistein as a low toxicity chemotherapeutic drug is hindered by many of its in vivo properties. To overcome these obstacles, a new multifunctional drug delivery system is developed, which is based on covalently attaching genistein onto Fe₃O₄ nanoparticles coated by cross-linked carboxymethylated chitosan (CMCH). The structure of the Fe₃O₄-CMCH-genistein nano-conjugate was confirmed by transmission electron micrographs (TEM), X-ray diffraction (XRD) and Fourier-transfer infrared (FT-IR) spectroscopy. The nano-conjugate shows good water solubility and superparamagnetic properties with a saturation magnetization of 55.1 emu/g. The effects of free genistein and Fe₃O₄-CMCH-genistein nano-conjugate on the proliferation and apoptosis of gastric cancer cell line SGC-7901 were investigated by MTT assay and flow cytometry (FACS). MTT results indicate that the Fe₃O₄-CMCH-genistein nano-conjugate exhibits a significantly enhanced inhibition effect to the SGC-7901 cancer cells than the free genistein. FACS data suggests that the inhibition on cell proliferation of the nano-conjugate is related with an induced apoptosis process. This drug delivery system is promising for future multifunctional chemotherapeutic application that combines drug release and magnetic hyperthermia therapy.

Keywords: FE3O4 NANOPARTICLES; GENISTEIN; DRUG DELIVERY; GASTRIC CANCER CELL LINE SGC-7901

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2010.1913

Publication date: 2010-04-01

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