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Multiplex Chemiluminescent Immunoassay Based on Silica Colloidal Crystal Beads

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Abstract:

The paper presented a novel multiplex chemiluminescence (CL) immunoassay method, which was based on silica colloidal crystals beads (SCCBs) as carriers using enhanced chemiluminescence (CL) detection of a horseradish peroxidase (HRP) catalyzing the luminal reaction. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. The experimental conditions were optimized and analytical performance was studied. Results showed that the SCCBs as supports were much sensitive (0.05 ng/ml IgG) than the glass beads (18 ng/ml IgG) and the planar carriers (125 ng/ml IgG). A multiplex immunoassay suggested the feasibility of high throughput applications. This novel immunoassay system provided a simple, sensitive and low-cost approach for multianalyte immunoassay without using of expensive array detector.

Keywords: CHEMILUMINESCENT IMMUNOASSAY; SILICA COLLOIDAL CRYSTALS BEADS; SUSPENSION ARRAY

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2009.1335

Publication date: November 1, 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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