Authors: Pei, Xiaoping; Zhao, Yuanjin; Zhao, Xiangwei; Xu, Hua; Chen, Baoan; Gu, Zhongze

Source: Journal of Nanoscience and Nanotechnology, Volume 9, Number 11, November 2009 , pp. 6320-6325(6)

Publisher: American Scientific Publishers

Abstract:

The paper presented a novel multiplex chemiluminescence (CL) immunoassay method, which was based on silica colloidal crystals beads (SCCBs) as carriers using enhanced chemiluminescence (CL) detection of a horseradish peroxidase (HRP) catalyzing the luminal reaction. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. The experimental conditions were optimized and analytical performance was studied. Results showed that the SCCBs as supports were much sensitive (0.05 ng/ml IgG) than the glass beads (18 ng/ml IgG) and the planar carriers (125 ng/ml IgG). A multiplex immunoassay suggested the feasibility of high throughput applications. This novel immunoassay system provided a simple, sensitive and low-cost approach for multianalyte immunoassay without using of expensive array detector.

Keywords: SILICA COLLOIDAL CRYSTALS BEADS; CHEMILUMINESCENT IMMUNOASSAY; SUSPENSION ARRAY

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2009.1335

Publication date: 2009-11-01

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