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Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

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Abstract:

We report the semi quantitative determination of the non specific adsorption of streptavidin onto raw and functionalized single walled nanotubes (SWNTs) using different approaches. SWNTs were covalently functionalized with carboxylated groups, amine groups and polyethylene glycol (PEG), and non-covalently functionalized with polyethylene glycol, Pluronic® F-127 and Tween20®. SWNTs covalent functionalization has been carried out through oxidation and acyl chloride formation. The non covalent functionalization was carried out by SWNTs dispersion in aqueous solution of the corresponding surfactant or polymer. A colorimetric test based on the horseradish peroxidase-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) was used to determine the presence of streptavidin onto the SWNTs. Streptavidin adsorption was also carried out by covalent bonding of biotin to SWNTs, to compare the specific and non-specific adsorption. Non specific streptavidin adsorption has been observed on pristine and all types of functionalized SWNTs tested. This adsorption of streptavidin on the surface of the SWNTs could be used to link other biomolecules to the nanotubes, using the high specific bonding between biotin and streptavidin.

Keywords: CARBON NANOTUBES; NON-SPECIFIC ADSORPTION; STREPTAVIDIN

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2009.1576

Publication date: October 1, 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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