Skip to main content

Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

Buy Article:

$113.00 plus tax (Refund Policy)

We report the semi quantitative determination of the non specific adsorption of streptavidin onto raw and functionalized single walled nanotubes (SWNTs) using different approaches. SWNTs were covalently functionalized with carboxylated groups, amine groups and polyethylene glycol (PEG), and non-covalently functionalized with polyethylene glycol, Pluronic® F-127 and Tween20®. SWNTs covalent functionalization has been carried out through oxidation and acyl chloride formation. The non covalent functionalization was carried out by SWNTs dispersion in aqueous solution of the corresponding surfactant or polymer. A colorimetric test based on the horseradish peroxidase-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) was used to determine the presence of streptavidin onto the SWNTs. Streptavidin adsorption was also carried out by covalent bonding of biotin to SWNTs, to compare the specific and non-specific adsorption. Non specific streptavidin adsorption has been observed on pristine and all types of functionalized SWNTs tested. This adsorption of streptavidin on the surface of the SWNTs could be used to link other biomolecules to the nanotubes, using the high specific bonding between biotin and streptavidin.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics


Document Type: Research Article

Publication date: 2009-10-01

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more