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Amperometric Biosensors Based on Biotinylated Single-Walled Carbon Nanotubes

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Abstract:

One challenging goal for the development of biosensors is the conception of three dimensional biostructures on electrode surfaces. In this context, single-walled carbon nanotube coatings (SWCNTs), functionalized by biotin groups, were investigated to develop 3D conductive nanostructures allowing a post-functionalization by biological macromolecules. This specific anchoring of biomolecules was carried via the affinity interactions using the avidin-biotin system. For this purpose, a biotinylated pyrene was specially synthesized to develop a non-covalent functionalization based on -interactions between pyrene and the nanotube sidewall. SWCNT coatings were also biotinylated via electropolymerization of biotin-pyrrole derivatives at 0.95 V in CH3CN electrolyte. The resulting biotinylated SWCNTs were modified by an avidin protein via affinity interactions and characterized with scanning electron microscopy. The biofunctionalization by a biotinylated glucose oxidase (GOX) was performed by successive incubation in avidin and GOX aqueous solutions via avidin bridges. The efficiency of the enzyme anchoring was examined through the electro-enzymatic activity of the modified electrodes towards the detection of glucose at 0.7 V versus SCE. The glucose sensitivity and maximum current density were 1.6 mAM−1cm−2 and 131 Acm−2 respectively for pyrene biotin-SWCNTelectrode and 2.5 mAM−1cm−2 and 178 Acm−2 respectively for the poly(pyrrole biotin)-SWCNT.

Keywords: AMPEROMETRY; BIOSENSOR; CARBON NANOTUBE; POLYPYRROLE; PYRENE

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2009.1549

Publication date: 2009-10-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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