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Direct Electron Transfer of Glucose Oxidase Immobilized on a Mesoporous Silica KIT-6 Matrix to Screen-Printed Electrodes

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The direct electron transfer of glucose oxidase (GOx) immobilized on a large-pore mesoporous silica KIT-6 matrix fixed to a screen-printed electrode (SPE) and its application as a disposable biosensor were studied. KIT-6 plays a vital role in this method through immobilizing GOx and facilitating the electron communication between the active centers of GOx and the surface of the SPE. GOx immobilized on KIT-6 maintains its bioactivity and structure and displays stable and well-defined redox peaks with a formal potential of −428 mV (vs. Saturated Calomel Electrode), with a heterogeneous electron transfer rate constant of 4.15 s−1 in phosphate buffer (0.05 M, pH 6.0). Upon the addition of glucose into air-saturated phosphate buffer solution, the reduction peak current decreases, which can be used analytically for glucose detection. The biosensor exhibits a linear response to glucose concentration ranging from 0 to 2.83 mM and a sensitivity of 7.29 μA (mM)−1 cm−2 at an applied potential of −0.5 V. The present work provides a promising strategy for fabricating a novel and disposable mediator-free glucose biosensor, which could be potentially mass-produced through further development.
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Keywords: DIRECT ELECTRON TRANSFER; ELECTROCHEMICAL BIOSENSOR; GLUCOSE OXIDASE; KIT-6; SCREEN-PRINTED ELECTRODE

Document Type: Research Article

Publication date: 01 August 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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