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Biocatalytic Activity of Fungal Protease on Silver Nanoparticle-Loaded Zeolite X Microspheres

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Silver nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. However, separation of the silver nanoparticle–enzyme bioconjugate material from the reaction medium is often difficult. In this study, we investigate the assembly of the silver nanoparticles on the surface of the amine-functionalized zeolite microspheres or formation of zeolite–silver nanoparticle "core–shell" structure and thereafter, using obtained structure in immobilization of fungal protease. The assembly of silver nanoparticles on zeolite surface occurs through the amine groups present in 3-aminopropyltrimethoxysilane (3-APTS). The fungal proteases bound to the massive "core–shell" structures can be easily separated from the reaction medium by mild centrifugation and exhibited excellent reuse characteristics. The biocatalytic activity of fungal protease in the bioconjugate was marginally enhanced relative to the free enzyme in solution.


Document Type: Research Article


Publication date: February 1, 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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