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Facile Preparation of Biopatternable Surface for Selective Immobilization from Bacteria to Mammalian Cells

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A facile route for cell patterning on functionalized surface with polyelectrolyte (PEL) and polyethylene glycol (PEG) microstructures is presented. Controlled cell adhesion is achieved by a micromolding in capillaries (MIMIC) on PEL coated surface, which produced two orthogonal regions including PEG region as physical and biological barriers to the nonspecific binding of cells and PEL surface for promoting cell adhesion. Our simple method but universal platform shows the first capable of versatile cell patterning from bacteria to animal cells. Although the adhesive proteins are necessary for the successful cell adhesion, our proposed method provide affordable surface for cell adhesion without adhesive proteins. In addition, the approach will be easily applied to various substrates such as glass, silicon, silicon oxide, and polymers.
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Keywords: CELL PATTERNING; MICROMOLDING IN CAPILLARIES; POLYELECTROLYTES

Document Type: Research Article

Publication date: 2009-02-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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