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The Fluorescence Recovery of Polydiacetylene/Fluorophore Vesicles by Interaction with Cetyltrimethylammonium Bromide

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Abstract:

Polydiacetylene (PDA) has been widely used as a biosensor candidate because it exhibits specific blue-red transition upon varied external stimulations, such as heat, pH, or mechanical pressure. We prepared mixed vesicles usingthe 10,12-pentacosadiynoic acid (PCDA) and fluorescence probe BO558, and investigated the interaction of surfactant with PCDA/BO558 vesicles. Experiments exhibited that the fluorescence of BO558 was significantly quenched owing to the ene-yne conjugated backbones in the PCDA vesicles. However, the fluorescence was gradually recovered in the presence of cetyltrimethylammonium bromide (CTAB) to the system. Meanwhile, the turbidity of the mixed solution decreased greatly with the increase of CTAB concentration. We investigated the mechanism of the fluorescence quenchingand recovery in the system of PCDA/BO558 vesicles and CTAB by UV-vis spectrum, stable fluorescence, transmission electron microscopy. The morphology of vesicles transformed with the change of surfactant concentration.

Keywords: FLUORESCENCE RECOVERY; POLYDIACETYLENE; SENSOR; SURFACTANT CTAB; VESICLES

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2009.C070

Publication date: February 1, 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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