Skip to main content

Fabrication of Recombinant Azurin Self-Assembled Layer for the Application of Bioelectronic Device

Buy Article:

$105.00 plus tax (Refund Policy)

A novel immobilization method of cupredoxin azurin on a gold surface was developed without a chemical linker using recombinant technique. A recombinant protein with cysteine residue by site-directed mutagenesis (SDM) was designed and then directly self-assembled on Au surface. The layer of the functionalized protein immobilized is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 73.2%, as compared to that of wild type azurin. The electrochemical property of the fabricated thin layer was investigated by the cyclic voltammetry (CV). As a result, cysteine-substituted azurin can be used for making high quality protein film, and applied to the fabrication of nano-scale bioelectronics.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: 2008-10-01

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more