Horseradish Peroxidase Immobilized on Poly(thiophene-2-aminophenol-3-thiopheneacetic acid) Film Electrode with Au Nanoparticle-Fabrication and Evaluation as Hydrogen Peroxide Sensor
Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by γ-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.
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Document Type: Research Article
Publication date: 2008-10-01
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