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Horseradish Peroxidase Immobilized on Poly(thiophene-2-aminophenol-3-thiopheneacetic acid) Film Electrode with Au Nanoparticle-Fabrication and Evaluation as Hydrogen Peroxide Sensor

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Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by γ-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.
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Keywords: ELECTROCHEMICAL REDUCTION; GAMMA-IRRADIATION; GOLD-NANOPARTICLE; HORSERADISH PEROXIDASE; POLYMER ELECTRODE

Document Type: Research Article

Publication date: 2008-10-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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