Immobilization, Hybridization and Amperometric Detection of DNA Sensor Using Quartz Crystal Microbalance
Authors: Lee, Dong-Yun; Choi, Won-Suk; Kafi, A. K. M.; Park, Sang-Hyun; Kwon, Young-Soo
Source: Journal of Nanoscience and Nanotechnology, Volume 8, Number 9, September 2008 , pp. 4553-4556(4)
Publisher: American Scientific Publishers
Abstract:A synthesized 21-mer single-stranded DNA (ssDNA) was covalently immobilized onto a self-assembled aminoethanethiol monolayer modified gold electrode onto quartz crystal microbalance (QCM). The covalently immobilized ssDNA was hybridized with complementary ssDNA. The interaction between surface-immobilized ssDNA and complementary 21-mer DNA in solution was also examined. Each step was followed by monitoring changes in the QCM frequency over time. From the results, the measured frequency change was about 256 Hz, 160 Hz and 141 Hz, respectively. As a result, the adsorption mass was about 273 ng/cm2, 171 ng/cm2 and 151 ng/cm2, according to the Sauerbrey equation. Also, PBS with pH 7.0 was selected as a supporting electrolyte to achieve maximum sensitivity and good bioactivity. The anodic peak current was linearly related to the concentrations between 1 × 10−5 and 9 × 10−5 M with a detection limit of 1.0 × 10 −6 M.
Document Type: Research Article
Publication date: September 2008
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- ingentaconnect is not responsible for the content or availability of external websites