Authors: Son, Ahjeong; Nichkova, Mikaela; Dosev, Dosi; Kennedy, Ian M.; Hristova, Krassimira R.

Source: Journal of Nanoscience and Nanotechnology, Volume 8, Number 5, May 2008 , pp. 2463-2467(5)

Publisher: American Scientific Publishers

Abstract:

We report application of lanthanide nanoparticles for DNA quantification in a microarray platform as a substitute for conventional organic fluorophores. A non-PCR based DNA microarray assay for quantifying bacteria capable of biodegrading methyl tertiary-butyl ether (MTBE) was demonstrated. Probe DNA was immobilized on a glass surface, hybridized with biotinylated target DNA and subsequently incubated with Neutravidin-biofunctionalized nanoparticles. The fluorescence spot intensities, measured by a commercial laser scanner, show a linear relationship (R² = 0.98) with bacterial 16S rDNA over a range of target DNA concentrations, while the background fluorescence remained low. In addition, nanoparticles fluorescence shows a stronger intensity than Quasar570 (Cy3). Present sensitivity of the assay is 10 pM of target DNA. The selectivity of the DNA-nanoparticle-probes to discriminate a non-target DNA with two base pairs mismatch in the 16S rDNA gene sequence was shown. The use of Eu:Gd₂O₃ nanoparticles as biolabels provides a relatively non-toxic, inexpensive, rapid and sensitive alternative to the materials currently used in DNA microarrays.

Keywords: NANOPARTICLES; LANTHANIDE EUROPIUM OXIDE; DNA MICROARRAYS; MTBE; METHYLIBIUM PETROLEIPHILUM PM1

Document Type: Short communication

DOI: http://dx.doi.org/10.1166/jnn.2008.347

Publication date: 2008-05-01

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