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Protein Arrays Based on Biotin-Streptavidin System for the Simultaneous Detection of TORCH Infections

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Abstract:

In this paper, we developed a protein array based on biotin-streptavidin system (PABS) used in the identification of IgM antibodies against TORCH antigens, including toxoplasma gondii (TOX), rubella virus (RuV), cytomegalovirus (CMV) and herpes simplex virus types II (HSV-2) antigens. The detection signal intensities and sensitivities between the PABS and the direct labeling array system (DLAS) were compared. The linear ranges of detectable IgM antibodies in PABS were 0.485–1000 g/mL, which was more sensitive than DLAS. Quantitatively, the lowest detectable amount for IgM antibodies on each spot of the PABS was 0.25 pg. Furthermore, sixty serum samples from patients were tested with the PABS in TORCH detection. All the results were correspondingly confirmed with ELISA assay. No significant differences in identifying TORCH specific IgM antibodies were found between the PABS and ELISA assay. There was a good concordance between PABS and ELISA in the classification of sera. The results suggested that the PABS was more sensitive, sample-saving and suitable for multi-pathogens parallel clinical detection.

Keywords: BIOTIN-STREPTAVIDIN SYSTEM; CYTOMEGALOVIRUS; HERPES SIMPLEX VIRUS; PROTEIN ARRAYS; RUBELLA; TOXOPLASMOSIS

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2008.276

Publication date: May 1, 2008

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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