Authors: Dorfman, Adam; Parajuli, Omkar; Kumar, Nitin; Hahm, Jong-in

Source: Journal of Nanoscience and Nanotechnology, Volume 8, Number 1, January 2008 , pp. 410-415(6)

Publisher: American Scientific Publishers

Abstract:

We developed a highly sensitive and effective telomeric repeat elongation (TRE) assay by exploiting the fluorescence signal enhancing ability of ZnO nanorod (NR) platforms. We report that this novel ZnO NR-based TRE assay can be successfully used for detecting active telomerase. Our TRE assays enable complex biological reactions between many different biological components to take place effectively on various ZnO NR platforms. Therefore, ZnO NRs can effectively determine telomerase activity even at ultratrace concentration level, by functioning as excellent fluorescence enhancing biosupports. We also show the potential of ZnO NR-based TRE assays in a high-throughput and multiplexed screening of active telomerase in a large number of samples. As telomerase is a useful biomarker in cancer diagnosis and screening, our ZnO NR-based TRE assays may prove to be not only useful in basic biological research but also in clinical testing.

Keywords: TELOMERASE; TELOMERIC REPEAT ELONGATION ASSAY; ZINC OXIDE NANOMATERIAL; FLUORESCENCE ENHANCEMENT; ZINC OXIDE BIOSENSOR

Document Type: Short communication

DOI: http://dx.doi.org/10.1166/jnn.2008.146

Publication date: 2008-01-01

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• Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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