Authors: Gong, Ping; He, Xiaoxiao; Wang, Kemin; Tan, Weihong; Xie, Wengang; Wu, Ping; Li, Huimin

Source: Journal of Nanoscience and Nanotechnology, Volume 8, Number 1, January 2008 , pp. 293-300(8)

Publisher: American Scientific Publishers

Abstract:

Rapid and sensitive detection of SARS associated coronavirus is critical for early diagnosis and control of severe acute respiratory syndrome. This study describes a method for the detection of SARS associated coronavirus gene by the combination of functionalized nanoparticles and PCR-based assay. In this method, the target cDNA of SARS associated coronavirus was firstly captured and enriched from the mixture of target cDNA and non-target cDNA by the use of the functionalized silica coated superparamagnetic nanoparticles. Additionally, the enriched target cDNA were amplified through a general symmetry PCR and then was selectively isolated from the double strands PCR products by applying the silica coated superparamagnetic nanoparticles again. Finally, we detected the amplified target cDNA by employing the functionalized silica coated fluorescent nanoparticles as signaling probes with a sandwich hybridization format. The results show that the target cDNA can be assayed successfully with a detection limit of 2.0 × 10³ copies and the nonspecific amplification could be inhibited. In addition, the detection procedure is rapid and can be completed in less than 6 h. Our results suggest that this approach will provide promising prospects for other pathogens detection.

Keywords: SARS-COV VIRUSES; PCR REACTION; DNA DETECTION; FUNCTIONALIZED SILICA COATED SUPERPARAMAGNETIC NAN; FUNCTIONALIZED SILICA COATED FLUORESCENT NANOPARTI; SANDWICH HYBRIDIZATION

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2008.010

Publication date: 2008-01-01

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• Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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