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Physicochemical Characterization of Densely Packed Poly(ethylene glycol) Layer for Minimizing Nonspecific Protein Adsorption

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Modification of the surface with densely packed poly(ethylene glycol) (PEG) brush layer was studied to improve the protein repellent ability of the surface. A PEG-brushed layer was constructed on a gold substrate using a PEG possessing a mercapto group at the chain end. The density of the PEG brushed layer substantially increased with repetitive adsorption/rinse cycles of the PEG on the gold substrate, allowing dramatic reduction of nonspecific protein adsorption. Notably, formation of a short, filler layer of PEG (2 kDa) in the preconstructed longer PEG brushed layer (5 kDa) achieved high density brush and almost complete prevention of nonspecific protein adsorption. On the other hand, surface modification with only long PEG chain (5 kDa) showed lower PEG brush density regardless of repetitive immobilization. Detailed characterization of the PEGylated surface was done from the physicochemical (QCM, contact angle, and SPR) as well as the biological (protein adsorption) point of view to highlight the relation between the PEG brush density and the protein repellent ability. Densely packed PEG surface which showed great protein repellent ability, presented in this study, suggests promising utility as engineered biomaterials including high-throughput screening and clinical diagnostics.
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Keywords: LOFSHITZ-VAN DER WAALS ATTRACTION; POLY(ETHYLENE GLYCOL); PROTEIN ADSORPTION

Document Type: Research Article

Publication date: 2007-07-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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