Rolled-up morphology of bilayer sheets in a self-assembled glycolipid nanotube (LNT) in water was carefully examined by using a cryogenic transmission electron microscope (cryo-TEM) with a rapid-freezing specimen-preparation technique. The LNTs were obtained under a series of self-assembly conditions: boiling of an aqueous dispersion of glycolipid N-(11-cis-octadecenoyl)--D-glucopyranosylamine, subsequent gradual cooling, and incubation at room temperature for several days. Cryo-TEM images revealed that the LNT walls consist of a multilayer structure with interlayer distance of about 4.7 nm. These layers correspond to constituent lipid bilayers. From the result of precise cryo-TEM observations and analyses, we confirmed the rolled-up morphology of the lipid bilayer sheets in a complete self-assembled glycolipid nanotube.
Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.