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Voltammetric Quantification of Adenine and Guanine at C60 Modified Glassy Carbon Electrodes

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Voltammetric determination of adenine and guanine has been carried out at C60 modified glassy carbon electrode at physiological pH. Well-defined oxidation peaks were observed with Ep at ∼990 mV and 692 mV for adenine and guanine respectively. Good colinearity was obtained in the concentration range 0.5 M to 100.0 M for adenine and guanine with sensitivity of ∼0.06 A M−1 and ∼0.03 A M−1, respectively. Simultaneous voltammetric determination of adenine and guanine has been described. Recovery studies for adenine and guanine in biological samples were also carried out. Interfering effect of some common metabolites including ascorbic acid has been evaluated. Analytical application of the developed protocol for determination of (G + C)/(A + T) ratio in DNA sample is also described.
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Document Type: Research Article

Publication date: 2006-12-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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