Direct Optical Visualization of DNA-DNA Interaction by Nanoparticle-Capture on Resonant PET-Films

Authors: Bauer, Maria; Haglmüller, Jakob; Pittner, Fritz; Schalkhammer, Thomas

Source: Journal of Nanoscience and Nanotechnology, Volume 6, Number 12, December 2006 , pp. 3671-3676(6)

Publisher: American Scientific Publishers

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Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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Abstract:

Based on the understanding of the absorption behavior of metal nanoparticles we aimed at the direct detection of sub-monomolecular layers of DNA with the naked eye. This extremely sensitive detection needs optical amplification techniques to be used in replacement of nanoparticle-aggregates applied e.g., in agglutination assays. We focus on the nanolayer-coated metallized-PET-chip setup and on the synthesis of DNA-nanoparticle conjugates suitable for 'resonance enhanced absorption'-point of care-tests and the application of those particles in the direct visualization of DNA-DNA binding events. Stabilization of nanoparticles and their sequence specific binding was proven with direct optical visibility of sub-monolayers of colored nanoclusters. Synthetic routes leading to suitable conjugates as well as stability tests and a biorecognition test are described in detail adding to the repertoire of tools that contribute to the application of nanoparticles in novel nano-enhanced devices.

Keywords: DNA-DNA INTERACTION; OPTICAL VISUALIZATION; NANOPARTICLE-CAPTURE

Document Type: Research article

DOI: 10.1166/jnn.2006.628