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Direct Optical Visualization of DNA–DNA Interaction by Nanoparticle-Capture on Resonant PET-Films

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Abstract:

Based on the understanding of the absorption behavior of metal nanoparticles we aimed at the direct detection of sub-monomolecular layers of DNA with the naked eye. This extremely sensitive detection needs optical amplification techniques to be used in replacement of nanoparticle-aggregates applied e.g., in agglutination assays. We focus on the nanolayer-coated metallized-PET-chip setup and on the synthesis of DNA-nanoparticle conjugates suitable for 'resonance enhanced absorption'-point of care-tests and the application of those particles in the direct visualization of DNA–DNA binding events. Stabilization of nanoparticles and their sequence specific binding was proven with direct optical visibility of sub-monolayers of colored nanoclusters. Synthetic routes leading to suitable conjugates as well as stability tests and a biorecognition test are described in detail adding to the repertoire of tools that contribute to the application of nanoparticles in novel nano-enhanced devices.

Keywords: DNA-DNA INTERACTION; NANOPARTICLE-CAPTURE; OPTICAL VISUALIZATION

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2006.628

Publication date: December 1, 2006

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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