If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

A Facile Synthesis and Physical Properties of Nano-Sized Dendritic α,ε-Poly(L-lysine)s for the Delivery of Nucleic Acids

$113.00 plus tax (Refund Policy)

Buy Article:

Abstract:

A series of nano-sized dendritic α,ε-poly(L-lysine)s (DPL) were synthesized by the solid-phase peptide synthesis method, using a core ε-peptide structure consisting of eight lysine residues. Surface amines of dendritic α,ε-poly(L-lysine) were characterized by comparing the retention times of a reverse phase HPLC with the electrophoretic mobilities of capillary zone electrophoresis (CZE) and non-denatured polyacrylamide gel electrophoresis (PAGE). The elution times of α,ε-poly(L-lysine) in HPLC were well correlated with the electrophoretic mobilities of CZE and PAGE. The separation was dependent on size, shape of molecule and the number of surface amine. The α,ε-poly(L-lysine) formed a complex with nucleic acids at various charge ratios and the degree of complex formation was size- and structure-dependent. Atomic force microscopy of the complex visualized the size and morphology of α,ε-poly(L-lysine)/DNA complex as a nano-sized spherical shape. The small size in complex formation provides a promise for in vivo therapeutic application of dendritic α,ε-poly(L-lysine)s or their derivatives in the delivery of gene or oligonucleotide.

Keywords: COMPLEX; DELIVERY; DENDRITIC POLYLYSINE (DPL); GENE

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2006.050

Publication date: November 1, 2006

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more