A Facile Synthesis and Physical Properties of Nano-Sized Dendritic α,-Poly(L-lysine)s for the Delivery of Nucleic Acids
Authors: Eom, Khee Dong; Kim, Jin Sook; Park, Sun Mi; Kim, Myong Soo; Yu, Rina; Jung, Hae Man; Kim, Saeng Gon; Yoo, Hoon
Source: Journal of Nanoscience and Nanotechnology, Volume 6, Number 11, November 2006 , pp. 3532-3538(7)
Publisher: American Scientific Publishers
Abstract:A series of nano-sized dendritic α,-poly(L-lysine)s (DPL) were synthesized by the solid-phase peptide synthesis method, using a core -peptide structure consisting of eight lysine residues. Surface amines of dendritic α,-poly(L-lysine) were characterized by comparing the retention times of a reverse phase HPLC with the electrophoretic mobilities of capillary zone electrophoresis (CZE) and non-denatured polyacrylamide gel electrophoresis (PAGE). The elution times of α,-poly(L-lysine) in HPLC were well correlated with the electrophoretic mobilities of CZE and PAGE. The separation was dependent on size, shape of molecule and the number of surface amine. The α,-poly(L-lysine) formed a complex with nucleic acids at various charge ratios and the degree of complex formation was size- and structure-dependent. Atomic force microscopy of the complex visualized the size and morphology of α,-poly(L-lysine)/DNA complex as a nano-sized spherical shape. The small size in complex formation provides a promise for in vivo therapeutic application of dendritic α,-poly(L-lysine)s or their derivatives in the delivery of gene or oligonucleotide.
Document Type: Research Article
Publication date: 2006-11-01
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