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A Facile Synthesis and Physical Properties of Nano-Sized Dendritic α,ε-Poly(L-lysine)s for the Delivery of Nucleic Acids

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Abstract:

A series of nano-sized dendritic α,ε-poly(L-lysine)s (DPL) were synthesized by the solid-phase peptide synthesis method, using a core ε-peptide structure consisting of eight lysine residues. Surface amines of dendritic α,ε-poly(L-lysine) were characterized by comparing the retention times of a reverse phase HPLC with the electrophoretic mobilities of capillary zone electrophoresis (CZE) and non-denatured polyacrylamide gel electrophoresis (PAGE). The elution times of α,ε-poly(L-lysine) in HPLC were well correlated with the electrophoretic mobilities of CZE and PAGE. The separation was dependent on size, shape of molecule and the number of surface amine. The α,ε-poly(L-lysine) formed a complex with nucleic acids at various charge ratios and the degree of complex formation was size- and structure-dependent. Atomic force microscopy of the complex visualized the size and morphology of α,ε-poly(L-lysine)/DNA complex as a nano-sized spherical shape. The small size in complex formation provides a promise for in vivo therapeutic application of dendritic α,ε-poly(L-lysine)s or their derivatives in the delivery of gene or oligonucleotide.

Keywords: COMPLEX; DELIVERY; DENDRITIC POLYLYSINE (DPL); GENE

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2006.050

Publication date: 2006-11-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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