Immobilization of Lysozyme onto Pore-engineered Mesoporous AlSBA-15

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Abstract:

In this research, hydrothermally stable mesoporous AlSBA-15 materials have been used as adsorbents for systematic research on the lysozyme adsorption. Stability of the AlSBA-15 adsorbents and the lysozyme molecules after the adsorption experiments for several days in aqueous solutions were confirmed by X-ray diffraction (XRD) measurement and FT-IR spectroscopy, respectively. The amount of the lysozyme adsorption can easily be controlled by the pore diameter and pore volume the mesoporous adsorbent, but an unreasonable effect of the surface area on the protein adsorption capacity was observed. The results of the effect of the pore diameter on the lysozyme adsorption suggest that the adsorption might partially be influenced by kinetically favorable edge-on type orientation on the confined mesopore. However, the final adsorption amount of the lysozyme can be well regenerated by models based on the side-on adsorption in dense packing. The present research also confirms the importance of appropriate "pore-engineering" for immobilization of bio-function on mesoporous materials.

Keywords: ALSBA-15; LYSOZYME; MESOPOROUS SILICA; PORE DIAMETER; PORE VOLUME; PROTEIN

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2006.221

Publication date: June 1, 2006

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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