Single Supramolecule Spectroscopy of Natural and Alkaline-Treated Chlorosomes from Green Sulfur Photosynthetic Bacteria
Authors: Saga, Yoshitaka; Wazawa, Tetsuichi; Ishii, Yoshiharu; Yanagida, Toshio; Tamiaki, Hitoshi
Source: Journal of Nanoscience and Nanotechnology, Volume 6, Number 6, June 2006 , pp. 1750-1757(8)
Publisher: American Scientific Publishers
Abstract:Fluorescence emission properties of intact and alkaline-treated chlorosomes containing bacterio-chlorophyll(BChl)-c, d, and e, which were isolated from four species of green sulfur photosynthetic bacteria, were successfully studied at the single-unit level using a total internal reflection fluorescence microscope. Single intact chlorosomes containing BChl-c from Chlorobium (Chl.) tepidum exhibited heterogeneous emission bands of BChl-c self-aggregates. In contrast, fluorescence spectra of chlorosomal BChl self-aggregates in single intact chlorosomes from the other three Chlorobium species were less heterogeneous than those from Chl. tepidum. Removal of energy-accepting BChl-a/protein complexes called baseplates from the intact chlorosomes by treatments with alkaline media hardly changed spectral shapes of BChl aggregates and their peak distributions at the single-chlorosome level. The similarity of spectral properties at the single-unit level between intact and alkaline-treated chlorosomes of four Chlorobium species clearly indicated that the removal of base-plates from intact chlorosomes by the alkaline-treatment did not affect BChl self-aggregates inside single chlorosomes.
Document Type: Research Article
Publication date: 2006-06-01
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