Authors: Xing, Xinli; He, Xiaoxiao; Peng, Jiaofeng; Wang, Kemin; Tan, Weihong

Source: Journal of Nanoscience and Nanotechnology, Volume 5, Number 10, October 2005 , pp. 1688-1693(6)

Publisher: American Scientific Publishers

Abstract:

Nanoparticles have seen wide applications in cellular research and development. One major issue that is unclear is the uptake of nanoparticles by cells. In this study, we have investigated the uptake of silica-coated nanoparticles by HeLa cells, employing rhoadime 6G isothiocyanate (RITC)-doped nanoparticles as a synchronous fluorescent signal indicator. These nanoparticles were synthesized with reverse microemulsion. A few factors, such as nanoparticle concentration, incubation time and temperature, and serum and inhibitors in culture medium were assessed on the nanoparticle's cellular uptake. The experimental results demonstrated that uptake was maximum after a 6 h incubation and was higher at 37 °C than that at 4 °C. Nanoparticle uptake depended on the nanoparticle concentration and was inhibited by hyperosmolarity, K⁺ depletion. In addition, serum in culture medium decreased the cellular uptake of nanoparticles. The results indicated that the uptake of silica-coated nanoparticles by HeLa cells was a concentration-, time-, and energy-dependent endocytic process. Silica-coated nanoparticles could be transported into HeLa cells in part through adsorptive endocytosis and in part through fluid-phase endocytosis.

Keywords: SILICA-COATED NANOPARTICLES; RITC; HELA CELL; UPTAKE; ENDOCYTOSIS

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2005.199

Publication date: 2005-10-01

More about this publication?

• Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
• Editorial Board
• Information for Authors
• Subscribe to this Title
• Terms & Conditions
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Technology
• By this author: Xing, Xinli ;  He, Xiaoxiao ;  Peng, Jiaofeng ;  Wang, Kemin ;  Tan, Weihong

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers