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Performance of an Amperometric Biosensor for the Determination of Hemoglobin

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Hemoglobin is a very important physiological index in human blood, especially for the evaluation of the physical performance of athletes. This paper reports an amperometric biosensor for the determination of blood hemoglobin, using potassium ferricyanide as a mediator, based on thin-film technology in a two-electrode system. The biosensor can be used for the determination of hemoglobin at concentrations in a linear range of 10–3000 M, and the correlation coefficient is 0.996, the relative standard deviation is 2.3% for six determinations for 2000 M hemoglobin concentration. And the linear range of hemoglobin is studied using methylene blue as a mediator.


Document Type: Research Article


Publication date: 2005-08-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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