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Expression of the Recombinant Fusion Protein CP15-23 of Cryptosporidium parvum and its Protective Test

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Abstract:

The CP15 and CP23 surface proteins on the sporozoite of Cryptosporidium parvum are major protective antigens. The recombinant plasmid pET28-15-23 was constructed based on the plasmids pMD18-T-15 and pMD18-T-23 with two pairs of specific primers using DNA recombinant technique. In the primers, a synthetic linker sequence encoding a peptide (G-G-S) was designed. After identification, the recombinant plasmids were transformed to component cells of Escherichia coli BL21 (DE3). The positive strain containing the recombinant plasmid could express a specific fusion protein (CP15-23, MW ≈ 25 kDa) induced by IPTG. The fusion protein could be recognized by the positive serum of mice infected with Cryptosporidium parvum oocysts specifically. The BALB/c mice were immunized with 80 g of CP15-23 protein 4 times at 2 week intervals. The mice produced specific antibodies that responded to the lysate of Cryptosporidium parvum oocysts and could prevent Cryptosporidium parvum infection. The results indicated that the recombinant fusion protein CP15-23 would be used as a candidate antigen to prevent cryptosporidiosis.

Keywords: CP15; CP23; CRYPTOSPORIDIUM PARVUM (C. PARVUM); FUSION PROTEIN; PROTECTIVE TEST

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2005.210

Publication date: August 1, 2005

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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