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Synthesis and Hybridization Studies of DNA on Functionalized Polypropylene Surfaces

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Abstract:

Polypropylene (PP) slice was treated with plasma in a mixture of nitrogen and hydrogen (1 : 2 V/V). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) demonstrated the successful grafting of amino groups. The as-treated slices were successively applied to the in-situ synthesis of oligonucleotides and an average coupling yield of more than 98% was achieved. The hybridization signals were recorded with fluorescent analysis system. The complementary and mismatched sequences were distinguished clearly, and the ratio of fluorescence intensity values (arbitrary units) for perfect match:single base mismatch:two bases mismatch:three bases mismatch is 108.6 : 62.9 : 22.4 : 5.5. The results implied the plasma-modified PP surface was extremely stable, performed well in DNA hybridization assays, and could service as a good substrate for high-density oligonucleotide array synthesis, especially in a multistep molecular stamping method to fabricate DNA microarrays in-situ on a large scale.

Keywords: DNA HYBRIDIZATION; IN-SITU SYNTHESIS OF OLIGONUCLEOTIDE; PLASMA TREATMENT

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2005.222

Publication date: 2005-08-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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